Three-Dimensional Super-Resolution in Eukaryotic Cells Using the Double-Helix Point Spread Function
نویسندگان
چکیده
منابع مشابه
Localizing and tracking single nanoscale emitters in three dimensions with high spatiotemporal resolution using a double-helix point spread function.
Three-dimensional nanoscale localization and tracking of dim single emitters can be obtained with a widefield fluorescence microscope exhibiting a double-helix point spread function (DH-PSF). We describe in detail how the localization precision quantitatively depends upon the number of photons detected and the z position of the nanoscale emitter, thereby showing a approximately 10 nm localizati...
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In large-scale spatial surveys, such as the forthcoming ESA Euclid mission, images may be undersampled due to the optical sensors sizes. Therefore, one may consider using a super-resolution (SR) method to recover aliased frequencies, prior to further analysis. This is particularly relevant for point-source images, which provide direct measurements of the instrument point-spread function (PSF). ...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2017
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2017.02.023